Introduction to next generation sequencing

2020

Taught biological researchers introductory programming and how to analyse next generation sequencing data.

The Introduction to NGS Data Analysis is a three-day, hands-on workshop that offers attendees a basic understanding of NGS data analysis workflows. The workshop provides hands-on computational experience in analysis of NGS data using common analytical approaches for QC, Alignment, RNA-Seq data and de novo genome assembly. This workshop was delivered using a mixture of lectures, hands-on practical sessions, and open discussions.

Topics covered by this workshop include:

• An Introduction to the command line interface and NGS file formats
• An introduction to R programming
• Quality control of sequencing data
• NGS read alignment
• Basic RNA-Seq differential expression analysis
• Short and long read de novo genome assembly

This workshop was developed by Bioplatforms Australia, in partnership with CSIRO, and with support from the European Bioinformatics Institute (EBI), a member of the European Molecular Biology Laboratory (EMBL) in the UK. The workshop content has been maintained and updated by a network of dedicated bioinformatics trainers from around Australia.